Regulating T-cell differentiation through the polyamine spermidine

BackgroundThe cross-talk between the host and its microbiota plays a key role in promotion of health. The production metabolites such as polyamines by intestinal-resident bacteria is part this symbiosis shaping immunity. putrescine, spermine, spermidine are abundant within gastrointestinal tract might substantially contribute to gut immunity.ObjectiveWe aimed characterize polyamine modulator T-cell differentiation function.MethodsNaive T cells were isolated from wild-type mice or cord blood healthy donors submitted polarizing cytokines, with without treatment, evaluate CD4+ vitro. Moreover, subjected oral supplementation spermidine, precursor l-arginine, assess frequency total numbers regulatory (Treg) vivo.ResultsSpermidine modulates vitro, preferentially committing naive phenotype. After activated lacking autophagy gene Atg5 fail upregulate Foxp3 same extent cells. These results indicate that spermidine's effect requires an intact autophagic machinery. Furthermore, dietary promotes homeostatic Treg reduces pathology model transfer-induced colitis.ConclusionAltogether, our highlight beneficial effects on immunity promoting cell development. We function. Naive vivo. Spermidine colitis. Altogether,